主办单位：中国气象局沈阳大气环境研究所
国际刊号：ISSN 1673-503X
国内刊号：CN 21-1531/P
气象与环境学报

气象与环境学报 ›› 2013, Vol. 29 ›› Issue (2): 107-112.doi:

• 快报 • 上一篇    

不同浓度化学融雪剂对草鱼抗氧化酶活性的影响

高群1,2  李法云2,3  范志平2  程志辉1,3  胡水1,2  张营1,3   

  1. 1.辽宁大学环境学院，辽宁 沈阳110036；2.辽宁石油化工大学生态环境研究院，辽宁 抚顺113001；3.辽宁省水环境生物监测与水生态安全重点实验室，辽宁 沈阳110036
  • 出版日期:2013-04-30 发布日期:2013-04-30

Effects of deicing salt of different concentrations on antioxidant enzymes activities in various organs of grass carp (ctenopharyngodon idellus)

GAO Qun1,2  LI Fa-yun2,3  FAN Zhi-ping2  CHENG Zhi-hui1,3  HU Shui1,2  ZHANG Ying1,3   

  1. 1. School of Environmental Science, Liaoning University, Shenyang 110036, China; 2. Institute of Ecoenvironmental Sciences, Liaoning Shihua University, Fushun 113001, China; 3. Liaoning Key Laboratory of Biological Monitoring for Water Environment and Water Eco-security, Shenyang 110036, China
  • Online:2013-04-30 Published:2013-04-30

PDF (PC)

589

被引次数

2

摘要： 化学融雪剂是寒冷地区冬季除雪的主要措施之一，其对地表水生态系统的累积性影响已逐渐受到重视。拟以草鱼为研究对象，采用邻苯三酚自氧化法和紫外吸收的方法，研究不同浓度融雪剂对鱼鳃和肌肉组织中SOD和CAT活性的影响，旨在为融雪剂对水生生物的毒性效应提供数据支持。在4.10、5.13、6.41、8.01 g·L-1和10.01 g·L-1不同浓度条件下，进行融雪剂对草鱼的暴露实验。结果表明：融雪剂对草鱼的96 h LC50值为14.19 g·L-1，安全浓度值应不超过4.03 g·L-1。根据化学融雪剂对草鱼（Ctenopharyngodon idellus）96 h LC50值，5.13、6.41、8.01、10.01 g·L-1浓度融雪剂暴露组与对照组相比，草鱼鳃和肌肉组织SOD的活性均被显著抑制 (P<0.05或P<0.01)。在7 d时，化学融雪剂胁迫下草鱼鳃和肌肉组织CAT活性均表现为显著抑制(P<0.05或P<0.01)，草鱼鳃中CAT活性高于肌肉组织中。

关键词: 融雪剂, 草鱼, 鳃, 肌肉, 超氧化物歧化酶, 过氧化氢酶

Abstract: Using deicing salt was one of the primary measures to remove the snow and ice in cold regions in winter, and its negative effect on ecosystem and environment has been gradually attracted attention. In order to detect the toxicity of deicing salt to aquatic organisms, the effects of deicing salt on superoxide dismutase (SOD) and catalase (CAT) activity in gills and muscle tissues of Ctenopharyngodon idellu (C.idellu) were investigated, with experimental methods of a pyrogallol autoxidation and a UV absorption spectrum. The exposure experiments of deicing salt on the C. idellus were carried out under five concentration treatments (4.10 g·L-1, 5.13 g·L-1, 6.41 g·L-1, 8.01 g·L-1 and 10.01 g·L-1), and the control treatment was set without deicing salt (0 g·L-1). The results show that the 96 h LC50 value of deicing salt for C. idellus is 14.19 g·L-1 and the safe concentration is 4.03 g·L-1. According to the 96 h LC50 value, the SOD activity in gills and muscle tissues of C. idellus is inhibited significantly under the concentrations of 5.13, 6.41, 8.01 g·L-1 and 10.01 g·L-1 (P<0.05 or P<0.01) compared with the control treatment. The CAT activity in gills and muscle tissues of C. idellus is inhibited significantly (P<0.05 or P<0.01) after 7 days comparing with the control treatment, and the CAT activity in gills of C. idellus is higher than that in the muscle tissues.

Key words: Deicing salt, Ctenopharyngodon idellus (C.idellu), Gills, Muscle, Superoxide dismutase (SOD), Catalase (CAT)