主办单位：中国气象局沈阳大气环境研究所
国际刊号：ISSN 1673-503X
国内刊号：CN 21-1531/P
气象与环境学报

气象与环境学报

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沈抚污灌区结冻土壤中微生物群落及石油烃优势降解菌的筛选

杨姝倩1;李素玉2;李法云1;谯兴国1;罗岩2;张志琼2   

  1. 1.辽宁大学环境科学系 沈阳110036;2.辽宁大学生命科学系 沈阳110036
  • 收稿日期:2006-04-13 修回日期:2006-05-08 出版日期:2006-06-01 发布日期:2006-06-01

Microbial population and screening of predominant strains for petroleum biodegradation in freezing soils of Shenfu wastewater irrigation area

YANG Shuqian1 LI Suyu 2 LI Fayun 1 QIAO Xingguo 1 LUO Yan 2 ZHANG Zhiqiong2   

  1. 1.Faculty of Environmental Science;Liaoning University;Shenyang 110036;2.Faculty of Biological Science;Liaoning University;Shenyang 110036
  • Received:2006-04-13 Revised:2006-05-08 Online:2006-06-01 Published:2006-06-01

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摘要： 针对已有50余年污水灌溉历史的沈抚污灌区石油污染结冻土壤中微生物种群及石油优势降解菌株进行了分离、筛选及初步鉴定。结果表明:石油污染土壤中的细菌总数是未污染土壤的100倍左右,其真菌和放线菌数量也明显超过未污染土壤。通过筛选,共得到17株石油烃优势降解细菌和11株真菌,其中,降解性能较强的细菌为12号,石油烃降解率为30.54%,初步鉴定为芽孢杆菌属(Bacillus);降解性能较强的真菌为22号真菌,石油烃降解率为34.21%,初步鉴定为常现青霉(Penicil-liam freguentans Westling)。

关键词: 石油污染土壤, 优势降解菌, 降解能力

Abstract: Microbial population and predominant strains for petroleum biodegradation were isolated and screened in freezing soils of Shenfu wastewater irrigation area with an irrigation history for more than 50 years.The results showed that the quantities of bacteria in the petroleum contaminated soil were about 100 times more than that in the non-contaminated soil,the quantities of fungus and ray fungus in the contaminated soil were also extensively more than that in the non-contaminated soil.17 bacteria strains and 11 fungus strains for petroleum biodegradation were screened from the contaminated soils.The petroleum biodegradation experiment represented that Bacillus 12~# and Penicilliam freguentans Westling 22~# had the highest capacity for petroleum removing,the removal rate of that was 30.54% and 34.21%,respectively.